significant changes in the activity of gabatransaminase and succinate semialdehyde dehydrogenase of mouse hypothalamus following peripheral injection of cholecystokinin-8 and/or caerulein

نویسندگان

r yazdanparast from the institute of biochemistry and biophysics, university of tehran

d qujeq the dept. biochemistry, tarbiat modarres university, tehran, islamic republic of iran.

چکیده

the activities of 4-aminobutyric-2-oxoglutaric acid transaminase (gaba-t) and succinate semialdehyde dehydrogenase (ssadh) were determined in mouse hypothalamus after peripheral injections of cholecystokinin-8 (cck-x)and/or caerulein (cln). gaba transaminase activity was measured utilizing endogenous succinate semialdellyde dehydrogenase to convert the product of gab a-t, succinate semialdehyde, to succinate. the concurrently formed nadh was used as an estimate of gaba-t activity. similarly, the activity of ssadh was determined in terms of nadh. injection of cck-8 and/or cln inhibited the gaba-t and ssadh activities in dose-dependent responses. the activities of gab a-tand ssadh were diminished by 52 and 66 percent respectively, 30 minutes after injection of cck-8 (50 µg/kg body weight).similarly, peripheral injection of cln (50 µg/kg body weight) reduced the activity of gaba-t and ssadh by 56 and 65 percent respectively, in 30 minutes. using ill vitro models, the full activities of gaba-t and/ or ssadh, after incubation with cck-8 and/or cln, were restored following 30 hours of dialysis at 4°c. these results indicate direct and reversible interactions between the catabolizing enzymes of gaba (gaba-t and ssadh) and the peptides cck-8 and cln.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

SIGNIFICANT CHANGES IN THE ACTIVITY OF GABATRANSAMINASE AND SUCCINATE SEMIALDEHYDE DEHYDROGENASE OF MOUSE HYPOTHALAMUS FOLLOWING PERIPHERAL INJECTION OF CHOLECYSTOKININ-8 AND/OR CAERULEIN

The activities of 4-aminobutyric-2-oxoglutaric acid transaminase (GABA-T) and succinate semialdehyde dehydrogenase (SSADH) were determined in mouse hypothalamus after peripheral injections of cholecystokinin-8 (CCK-X)and/or caerulein (CLN). GABA transaminase activity was measured utilizing endogenous succinate semialdellyde dehydrogenase to convert the product of GAB A-T, succinate semiald...

متن کامل

Significant Changes in the Activity of L-Glutamic Acid Decarboxylase of Mouse Hypothalamus After Peripheral Injection of Cholecystokinin-8 and Caerulein

The activity of one of the metabolizing enzymes of - aminobutyric acid, (GABA), was determined in mouse hypothalamus after peripheral injections of cholecystokinin-8 (CCK-8) and caerulein (CLN). The activity of this rate-limiting enzyme, L-glutamic acid decarboxylase, (GAD), did not change thirty minutes after peripheral injections of either CCK-8 or CLN in doses of 50g/kg body weight. Howeve...

متن کامل

significant changes in the activity of l-glutamic acid decarboxylase of mouse hypothalamus after peripheral injection of cholecystokinin-8 and caerulein

the activity of one of the metabolizing enzymes of - aminobutyric acid, (gaba), was determined in mouse hypothalamus after peripheral injections of cholecystokinin-8 (cck-8) and caerulein (cln). the activity of this rate-limiting enzyme, l-glutamic acid decarboxylase, (gad), did not change thirty minutes after peripheral injections of either cck-8 or cln in doses of 50g/kg body weight. howeve...

متن کامل

Significant Changes in the Activity of L-glutamic Acid Decarboxylase of Mouse Hypothalamus after Peripheral Injection of Cholecystokinin-8 and Caerulein

The activity of one of the metabolizing enzymes of aminobutyric acid, (GABA), was determined in mouse hypothalamus after peripheral injections of cholecystokinin-8 (CCK-8) and caerulein (CLN). The activity of this rate-limiting enzyme, L-glutamic acid decarboxylase, (GAD), did not change thirty minutes after peripheral injections of either CCK-8 or CLN in doses of 50g/kg body weight. However,...

متن کامل

comparison of catalytic activity of heteropoly compounds in the synthesis of bis(indolyl)alkanes.

heteropoly acids (hpa) and their salts have advantages as catalysts which make them both economically and environmentally attractive, strong br?nsted acidity, exhibiting fast reversible multi-electron redox transformations under rather mild conditions, very high solubility in polar solvents, fairly high thermal stability in the solid states, and efficient oxidizing ability, so that they are imp...

15 صفحه اول

the analysis of the role of the speech acts theory in translating and dubbing hollywood films

از محوری ترین اثراتی که یک فیلم سینمایی ایجاد می کند دیالوگ هایی است که هنرپیش گان فیلم میگویند. به زعم یک فیلم ساز, یک شیوه متأثر نمودن مخاطب از اثر منظوره نیروی گفتارهای گوینده, مثل نیروی عاطفی, ترس آور, غم انگیز, هیجان انگیز و غیره, است. این مطالعه به بررسی این مسأله مبادرت کرده است که آیا نیروی فراگفتاری هنرپیش گان به مثابه ی اعمال گفتاری در پنج فیلم هالیوودی در نسخه های دوبله شده باز تولید...

15 صفحه اول

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
medical journal of islamic republic of iran

جلد ۷، شماره ۴، صفحات ۲۶۳-۲۶۸

کلمات کلیدی
[ ' c a e r u l e i n . c h o l e c y s t o k i n i n ' , 8 , ' c a t a b o l i s m . g a b a ' , ' t r a n s a m i n a s e ' , ' s u c c i n a t e s e m i a l d e h y d e ' ]

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023